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[13] For the liver side specifically, researchers use hepatocyte lipid-loading models (exposing primary human hepatocytes or HepG2 cells to fatty acids such as oleate and palmitate to induce steatosis in a dish), hepatic stellate-cell lines (for example LX-2) to study fibrogenic activation, and increasingly three-dimensional systems — liver organoids, spheroids, and liver-on-a-chip devices — that better reproduce cell–cell interactions

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Regulating appetite through dual hormone signaling